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In the next step of the one-carbon cycle, SAM donates its methyl group to a variety of acceptor molecules, leading to S-adenosylhomocysteine (SAH) formation ( Figure 1 ) (Lu, 2013)

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Each sgRNA was cloned into the PX459 vector and transfected separately into HCC cells using Lipofectamine 3000 (ThermoFisher)

Cancer 62:174183 Nguyen CT, Tan J, Blackwell KE, Bhuta S, Sercarz JA (2000) Primary melanocytic schwannoma of cervical sympathetic chain